My scientific path began in 2005 as an undergraduate Biochemistry student at University of Algarve. I have worked on the development of a phage display system in order to targeting cancer cells as an alternative powerful tool for cancer diagnosis. The aim of this project was to identify proteases that were differentially expressed at the surface of several types of malignant cell lines, using combinatorial libraries of phage particles displaying protease inhibitor peptides.
In 2008 I have started my master thesis in Biomedical Sciences and join Protein Degradation Lab at CBME to study proteasome assembly, in collaboration with Dohmen’s Lab at Institute of Genetics at Cologne (Germany) where I did part of my work. By then, I was investigating the early stages of proteasome biogenesis, using S. cerevisiae as a biological model. The goal was to clarify some aspects concerning the mode of action of Ump1 and Poc1-Poc2 (dedicated assembly chaperones) in the assembly of 20S proteasome. During this work, I was able to use molecular and biochemical tools combined along with in vitro binding assays.
After finishing my master thesis I was interest to proceed and improve my skills in the Biomedical research area, so I applied to Rui Martinho’s lab in 2012. I joined the group as a research trainee and lab manager. Currently, I am collaborating in several tasks related to the project “Regulation of protein N-terminal acetylation during Drosophila development”.